In this study, 36 ga derivatives were synthesized and screened in a zebrafish model to evaluate their antiangiogenic activity and toxicity. Targeting cancer using polymeric nanoparticle mediated. We modified the antiegfrirgd recombinant protein on the surface of rbcmnps by lipid insertion method to construct ierbcmplga nps and confirmed the presentation of active tumortargeting ability in colorectal cancer models with high egfr expression when compared with rbcmplga nps. Inclusion complex from cyclodextringrafted hyaluronic. It has been shown that the size of the nanoparticle is a major factor determining the in vivo fate of the particles. Nanomedical approaches to drug delivery center on developing nanoscale particles or molecules to improve drug bioavailability.
These parameters affect the structure of the initial gel and, in turn, the properties of the material at all subsequent processing steps. Ga targets multiple biological events in the inhibition of cancer cells. The term nano is derived from the greek word nanos which means small and it is used as the prefix for one billionth part 109. Gold chloride mm, ascorbic acid mm and gum arabic 1% wv solutions were prepared. Synthesis and characterization of zno nano particles submitted by jayanta kumar behera in partial fulfillment for the award of the degree of master of science in physics under the esteemed guidance of dr. Gambogic acid ga, a natural compound from gamboge resin that produced by genus garcinia trees in asia and africa, is reported to exhibit significant cytotoxic activity against multiple types of tumor including breast carcinoma, melanoma, leukemia, lung carnicoma, etc. As a good photosensitizer, tio2 nanomaterials show potential biomedical applications, such as drug carriers or enhancers in photodynamic therapy. Herein, to meet the need of the submitted work investigates gold nanohigh concentrations, we synthesized uniform gnps particles synthesis by reduction reaction between from 10, 15, 20 mm haucl 4 solutions. Gambogic acid augments black phosphorus quantum dots bpqds. Derivatives 4, 32, 35, 36 effectively suppressed the formation of newly grown blood vessels and showed lower toxicities than ga as evaluated by zebrafish heart.
Antitumor studies with peptidedecorated polymeric nano medicines. It effectively covers chemical synthesis and precisely. In recent decades, interest in gambogic acid as a potential anticancer agent has increased because it demonstrates antitumor, antiangiogenic, and antimetastatic activities against multiple cancer cell lines. As particles shrink in size there is a dramatic change in these bands, as the continuous density of states in bulk is replaced with a set of discrete energy levels. Natural compounds have been known as biosafety agents for their significant clinical and biological activity against dreadful diseases, including cancer, cardiovascular, and neurodegenerative disorders. Nov 25, 2016 gambogic acid ga is extracted from gamboge and the monomers are purified. However, the poor aqueous solubility and short halflife hinder its clinical application. Anticd22conjugated cdte qds coloaded with doxorubicin and. After gelation, the wet gel can be optionally aged in its mother liquor, or in another solvent, and washed. Modification of this method is known as highpressure emulsification and solvent evaporation method jaiswal et al.
Conditions for a and b as in figure 1a and b respectively. Nanomedicine 287 11 designing polymeric nanoparticles for targeted drug delivery system ida idayu muhamad12, suguna selvakumaran1, nurul asmak md lazim1 1bioprocess engineering department, faculty of chemical engineering, universiti teknologi malaysia johor bahru, malaysia 2cardiovascular engineering centre ijnutm, faculty of bioscience and medical engineering, universiti teknologi. The molecular formula of ga is c 38 h 44 o 9 molecular weight, 628. Studies on lactoferrin nanoparticles of gambogic acid for oral delivery zhenhai zhang 1,2, xiaopan wang 1, waddad y. Gambogic acid is a xanthonoid that is derived from the brownish or orange resin from garcinia hanburyi. The particle size, polydispersity index pdi and zeta potential of the particles were measured by dynamic light scattering dls at 25 c using nano series nano zs90 zetasizer malvern instruments ltd, malvern, uk. The current methods for targeted drug delivery utilize ligands that must outcompete endogenous ligands in order to bind to the active site facilitating the. Pdf studies on lactoferrin nanoparticles of gambogic acid for oral. Phytoconstituents as pharmacotherapeutics in rheumatoid arthritis. Gambogic acidloaded electrosprayed particles for site. Gambogic acidloaded biomimetic nanoparticles in colorectal. Anticd22conjugated cdte qds coloaded with doxorubicin. This study aims to assess the targeted effect and antitumor efficacy of gambogicacidloaded particles gaps. Escherichia coli bl21 de3, which express recombinant proteins antiegfrirgd, was preserved by our laboratory.
Phytoconstituents as pharmacotherapeutics in rheumatoid. Recent studies showed release of nanotio 2 from the outer layer of exterior facades through water leaching 9. Pdf studies on lactoferrin nanoparticles of gambogic acid. Full text gambogic acidloaded biomimetic nanoparticles.
Ga is an effective antitumor agent that has been shown to have multiple effects on several types of solid human tumors in vitro and in vivo 14, 15. Gambogic acidlactoferrin nanoparticles glnps were prepared by the nanoparticle albuminbound nab technology. The resulting mixture was stirred overnight at room temperature. The particle size, polydispersity index, encapsulation efficiency and loading capacity of. Synthesis and antiangiogenic activity of novel gambogic acid. In this contribution, novel nanocomposites through the blending of tio2 nanofibers with the active compound, gambogic acid ga, were explored, and the results showed that ga could inhibit cancer cell proliferation in a timedependent and dose. Gambogic acid ga, a naturally occurring xanthonebased moiety, reported from garcinia hanburyi tree, is known to perform numerous intracellular and extracellular actions, including programmed. Garcinia hanburyi is a small to mediumsized evergreen tree up to approximately 15 m in height with smooth grey bark.
Designing polymeric nanoparticles for targeted drug. Pluronic f68linoleic acid nanospheres mediated delivery. Gambogic acid ga, a natural compound from gamboge resin, has been introduced as a promising antitumor drug contributing to its broad spectrum of antitumor activity. Abstractpoor water solubility and side effects hampered the clinical application of gambogic acid ga in cancer therapy. Gambogic acid betaguttiferrin bcl2 family inhibitor. Gambogic acidloaded pegpcl nanoparticles act as an. Understanding resonant lighttriggered dna release from. The nanosize range of these delivery systems allows them to be injected directly into the systemic circulation without the risk of blocking blood vessels.
Gambogic acid ga, a natural compound from gamboge resin that produced by genus garcinia trees in asia and africa, is reported to. Thousands of new, highquality pictures added every day. Pluronic f68linoleic acid nanospheres mediated delivery of. It is native to cambodia, southern vietnam, and thailand and has been successfully introduced in singapore. The appearance of gambogic acid at various dosage forms was examined. Gambogic acid ga is the main active ingredient of gamboge resin that. The nano particles are collected by ultracentrifugation and washed with distilled water to remove stabilizer residue or any free drug and lyophilized for storage song et al. Nanotechnology offers an immense assortment of novel applications, most notably the use of nanomaterials as.
Nanotechnology offers an immense assortment of novel applications, most notably the use of nanomaterials as scaffolds. Gambogic acidloaded biomimetic nanoparticles in colorectal cancer treatment. These devices are faster and more sensitive than typical drug delivery 32. Find nanoparticles stock images in hd and millions of other royaltyfree stock photos, illustrations and vectors in the shutterstock collection. Singlewalled carbon nanotube and graphene nanodelivery of.
Chapter iii 3 introduction to synthesis of nanomaterials. To improve therapeutic selectivity, one important strategy is to remotely trigger the release of a therapeutic cargo from a specially designed gene or drugladen nearinfrared nir absorbing gold nanoparticle complex with nir light. Gambogic acid ga is in a phase ii clinical trial as an antitumor and antiangiogenesis agent. The activity of gambogic acid against the 6 human antiapoptotic bcl2family proteins is contrasted, using fpas. Size controlled synthesis of gold nanoparticles using.
Optical properties this leads to interesting optical properties. A novel dual sensitive polymergambogic acid conjugate. The reduction of a tetrachloroauric acid haucl 4 has been initiated by trisodium citrate na 3 c 6 h 5 o 7 by injecting specified amount of preheated trisodium citrate solution to a boiled gold solution in a double walled reactor, which is heated by a bath thermostat in order to prevent the presence of temperature gradients in the liquid. But when a small amount of seed particles was added. Pdf gambogic acidloaded biomimetic nanoparticles in.
Although a positive surface charge contributed towards a higher particle uptake, this study did not evaluate the cationic chargerelated cytotoxicity. Inducing cell cycle arrest and apoptosis by dimercaptosuccinic acid modified fe 3 o 4 magnetic nanoparticles combined with nontoxic concentration of bortezomib and gambogic acid in rpmi8226 cells. Gambogic acidloaded biomimetic nanoparticles in colorectal cancer treatment zhen zhang,1 hanqing qian,2 mi yang,2 rutian li,2 jing hu,1 li li,1 lixia yu,2 baorui liu,1,2 xiaoping qian1,2 1comprehensive cancer center, nanjing drum tower hospital clinical college of traditional chinese and western medicine, nanjing university of traditional chinese medicine, 2comprehensive cancer center. In this study, lf nanoparticles were firstly used as carriers of gambogic acid ga to enhance oral absorption and anticancer activity, hence. Jul 22, 2016 natural compounds have been known as biosafety agents for their significant clinical and biological activity against dreadful diseases, including cancer, cardiovascular, and neurodegenerative disorders. Nanoparticle delivery and combination therapy of gambogic acid.
Oct 24, 2016 gambogic acid ga is polyprenylated xanthone containing resin which is derived from garcinia hanburyi and garcinia morella. Anticd22conjugated cdte qds coloaded with doxorubicin and gambogic acid. Clusiaceae and has been used for centuries to treat infections and tumors ren et al. Mucoadhesive nanoparticles based on ros activated gambogic. Cytotoxicity was assessed by measuring lactate dehydrogenase release, mitochondria dehydrogenase activity, mitochondrial membrane.
Gambogic acid augments black phosphorus quantum dots. Silver nanoparticles could also affect whole societies and ecosystems silver nanoparticles can inhibit many bacteria, including good bacteria silver nanoparticles can prevent photosynthesis in algae societal and ethical implications of nano silver. Ga is an effective antitumor agent that has been shown to have multiple effects on several types of solid human tumors in vitro and in vivo 14,15. This study aims to assess the targeted effect and antitumor efficacy of gambogic acid loaded particles gaps. Natural products derived from plants, animals or microbes, have become one of the major sources for developing antitumor therapeutics, by virtue of their evolution to preferentially interaction with the molecular targets in biological system. Spherical pure fe3o4sio2 nanoparticles with an effective dynamic diameter of 230 nm were also prepared for comparison. In bottom up process the nanoparticles are built by joining atoms, molecules and smaller particles 3 as shown in figure 1. Pdf zhen zhang,1 hanqing qian,2 mi yang,2 rutian li,2 jing hu,1 li li,1 lixia yu,2 baorui liu,1,2 xiaoping qian1,2 1comprehensive cancer center. Gambogic acidloaded magnetic fe3o4 nanoparticles inhibit.
In this study, we report the proteomewide profiling of potential cel. Inclusion complex from cyclodextringrafted hyaluronic acid. The system showed appropriate diameter, good dispersibility, high encapsulation e. Gambogic acidloaded magnetic fe3o4 nanoparticles inhibit panc1 pancreatic cancer cell proliferation and migration by inactivating transcription factor.
Gambogic acid has documented cytotoxic activity against tumor cell lines in culture, with concentrations required for killing 50% of cells ld 50 of 1. Modification of this method is known as highpressure emulsification and solvent evaporation method jaiswal et. Studies on lactoferrin nanoparticles of gambogic acid for. Antiegfrirgd recombinant protein modified biomimetic. The drug is dissolved, entrapped, encapsulated or attached to a nanoparticle matrix. Nanocapsules are systems in which the drug is confined to a. In this study, lf nanoparticles were firstly used as carriers of gambogic acid ga to enhance oral absorption and anticancer activity, hence reducing the related.
Tem images of the synthesized gold nanoparticles fig. Nanoparticlebased platforms for gene therapy and drug delivery are gaining popularity for cancer treatment. Gambogic acid ga, a naturally occurring xanthonebased moiety, reported from garcinia hanburyi tree, is known to perform numerous intracellular and extracellular actions, including. According to american society for testing and materials astm international 2006, nanoparticles are those particles which have two or more than two.
Synthesis and antiangiogenic activity of novel gambogic. The system, designated as doxgacdtecd22, can be used for nanoparticlebased targeted combination chemotherapy. Enms are applied in many commercially available consumer products such as cosmetics, textiles and paints. A novel exploration of a combination of gambogic acid with. Ascorbic acid was used for the reduction of gold chloride. Gambogic acid has shown significant potential as an anticancer and antiinflammatory compound. Gambogic acid betaguttiferrin is a medicinal compound derived from the gamboges resin of the tree, garcinia hanburyi. Chemical formulae of gold chloride and ascorbic acid are haucl 4 3h 2o and c 6h 8o 6 respectively. This route is more often used for preparing most of the nanoscale materials with the ability to generate a uniform size, shape and distribution. Global profiling of cellular targets of gambogic acid by. Improving aqueous solubility and antitumor effects by. Synthesis and characterization of zno nanoparticles. Graphene and singlewalled carbon nanotubes were used to deliver the natural lowtoxicity drug gambogic acid ga to breast and pancreatic cancer cells in vitro, and the effectiveness of this complex in suppressing cellular integrity was assessed. Rational design of gomodified fe3o4sio2 nanoparticles.
Nanoparticles are currently made out of a very wide variety of materials, the most common of the new generation of nanoparticles being ceramics, which are best split into metal oxide ceramics, such as titanium, zinc, aluminum and iron oxides, to name a prominent few, and silicate nanoparticles silicates, or silicon oxides, are also. Peptidedecorated polymeric nanomedicines for precision cancer. Studies on lactoferrin nanoparticles of gambogic acid for oral delivery. Gambogic acidloaded biomimetic nanoparticles in colorectal cancer treatment zhen zhang,1 hanqing qian,2 mi yang,2 rutian li,2 jing hu,1. Mucoadhesive nanoparticles based on ros activated gambogic acid prodrug for safe and efficient intravesical instillation chemotherapy of.
Pluronic f68 f68 is a wellknown amphiphilic block copolymer consisting of hydrophobic propylene oxide units and hydrophilic ethylene. Dillip kumar bisoyi department of physics national institute of technology, rourkela769008, orissa, india. Depending upon the method of preparation, nanoparticles, nanospheres or nanocapsules can be obtained. The utility of nanotechnology in medicine, specifically within the field of orthopedics, is a topic of extensive research. The current methods for targeted drug delivery utilize ligands that must outcompete endogenous ligands in order to bind to the active site facilitating the transport.
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